国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1949

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 04.03 (ATCC® CRL-2555™)

Organism  Homo sapiens, human

Tissue  pancreas

Product Format  frozen

Morphology  epithelial

Culture Properties  adherent

Biosafety Level  1

Disease  adenocarcinoma

Age  70 years adult

Gender  male

Ethnicity  White

Storage Conditions  liquid nitrogen vapor phase

Derivation  Panc 04.03 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Clinical Data  70 years

Caucasian

male

Antigen Expression  MHC class I +; MHC class II -

RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Blood type O; Rh+

Oncogene  K-ras + RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Genes Expressed  cytokeratins 7 and 18 RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Tumorigenic  Yes

Effects  Yes, forms tumors in nude or SCID mice

Comments  The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

The cells have a reported plating efficiency of 80%. Ref

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

•20 Units/ml human recombinant insulin

•fetal bovine serum to a final concentration of 15%


Subculturing  Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: 1:2 to 1:3

Medium Renewal: Add media once per week. Fluid change one to two times per week.


Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation  Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions  Temperature: 37°C




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产成人精品白浆久久69| 中文无码AV一区二区三区| 波多野结衣电影| √天堂中文官网8在线| 亚洲日产乱码一二三区别| 熟妇高潮喷沈阳45熟妇高潮喷| 97色偷偷色噜噜狠狠爱网站| 欧美vpswindows另类| 少年阿宾1一72全文目录| 最近2019中文字幕大全视频1| 50多岁岳不让我戴套| 亚洲 日韩 欧美 综合 热| 免费无码一区二区三区蜜桃| gogogo高清在线播放| 少妇中文字幕乱码亚洲影视 | 精品人妻伦一二三区久久| 噜噜噜精品欧美成人av| 国产手机在线精品| 人妻丝袜无码国产一区| 成人做爰视频www| 国产乱码一区二区三区| 好男人好资源影视在线| 久久99国产精品久久| 偷偷藏不住小说免费阅读| 波多野结衣中文字幕一区二区三区| 亚洲一区二区三区香蕉| 丰满大爆乳波霸奶| 亚洲午夜无码AV毛片久久| 护士趴下光屁股翘臀被打的作文| 囯产极品美女高潮无套久久久 | 日韩毛片无码永久免费看| 亚洲av午夜福利精品香蕉麻豆| 惩罚她给她用最粗的玉势h| 第一次破了女处小红| 成人免费视频在线观看| 国产日产欧产综合| 天堂А√在线地址中文在线| 国模吧无码一区二区三区| 把女人弄爽的特黄A大片| 少妇放荡的呻吟干柴烈火| 麻豆蜜桃国产精品无码视频|