国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2547Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

簡(jiǎn)要描述:Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2547
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:1577

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

ATCC® Number:CRL-2547™    Price:$338.00
Dsafety Level:1Shipesignations:Panc 10.05Depositors:EM JaffeeBioped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞Isolation:Isolation date: 1992Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II -DNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9,12 D5S818: 13 D7S820: 8,9 THO1: 6,9.3 TPOX: 11 vWA: 16Age:*****Gender:maleEthnicity:WhiteComments:Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma. Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 40%. [50655] The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558).Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Temperature: 37.0°CSubculturing:Protocol:                        Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:19.2 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020derived from same individual:ATCC CRL-2558References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
美人双性受h多人运动np| 欧美激情综合五月色丁香| 18禁老湿机体验区试看120秒| 亚洲欧美日本韩国| 久久精品国产久精国产| 汽车上开两个的花苞| 久久8精品亚洲AV无码| 免费A级毛片无码A∨免费软件 | 超碰免费公开| 香蕉欧美成人精品av在线| 天天躁日日躁AAAAXXXX| 国产日韩未满十八禁止在线观看| 厨房解开奶罩吸奶头高潮小说| 久久精品国产精品亚洲| 亚洲日韩精品一区二区三区| 大肥波bbwwhbbww| 亚洲AV无码一区二区一二区| 粗大猛烈进出高潮视频大全 | chinesevideo国产熟妇| 亚洲人成色7777在线观看不卡| 日日av夜夜添久久奶无码| 少妇wwb搡bbbb搡bbbb| 狠狠色综合TV久久久久久| 97高清视频在线观看免费| 日韩精品视频| 精品国产成人亚洲午夜福利| 俄罗斯引擎yandex网站| 春药play揉屁股play男男| 男男做爰猛烈叫床视频gv| 99热在线观看| 久久精品夜夜夜夜夜久久| 2021少妇久久久久久久久久| 污调教贱乳打屁股扒开作文| 我们的日子电视剧免费观看完整版| 中文字幕在线播放| 国产精品毛片一区二区| 国产AV一区二区三区传媒| 女人荫蒂添的好舒服a片| 散装肉脯(h)海棠书屋| 亚洲精品久久久久久| 国产乱子伦农村叉叉叉|