国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1622KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

簡(jiǎn)要描述:KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1622
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1791

產(chǎn)品分類

Product Category

詳細(xì)介紹

KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

ATCC® Number:CRL-1622™    Price:$338.00
Designations:KLE

Depositors:GR Richardson

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:


Source:Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

Tumorigenic:Yes

Antigen Expression:blood type O; Rh+

DNA Profile (STR):Amelogenin: X

CSF1PO: 13,14

D13S317: 12

D16S539: 11,12

D5S818: 9,12

D7S820: 11,12

THO1: 6,7

TPOX: 8,11

vWA: 16



Age:64 years adult

Gender:female

Ethnicity:Caucasian

Comments:Electron microscopy of tumors formed in nude mice shows microvilli and junctional complexes, and nucleolar channel systems are present that are similar to those seen in normal endometrium under progestational stimulation. The tumors do not form glands.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:                    
  1. Remove and discard cult   ure medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久人人爽人人爽人人AV | 极度另类freesex强行真实| 亚洲成色WWW久久网站| 久久久久精品日韩久久久| 成免费crm| 两个人看的WWW高清视频中文| 国产黄大片在线观看| 国产无遮挡无码视频免费软件| 国产日韩欧美一区二区三区| 国产精品乱码一区二三区 | 把腿张开老子臊烂你| 图片区小说区另类春色视频| 人妻巨大乳hd免费看| 国产AV熟女一区二区三区| 性欧美乱熟妇xxxx白浆| 国产精品毛片久久久久久久| 无码人妻精品中文字幕免费东京热| 成全视频在线观看免费| А天堂中文最新版在线官网| 久久精品国产| 国产精品久久久久久亚洲AV| 又硬又粗又长又爽免费看| 罗曼史在线观看| 国产在线一区二区三区AV| 97精品人人a片免费看| 无码人妻少妇伦在线电影| 久久久久久AV无码免费看大片| 金瓶悔1一5扬思敏完整版| 国产成人av无码一二三区| 欧VODAFONEWIFI69| 97人妻精品一区二区三区 | 狠狠综合久久AV一区二区三区| 国产亚洲精品无码成人| 国内精品久久久久久久97牛牛| 久久国产精品-国产精品| 欧美xxxxx高潮喷水麻豆| 少妇被粗大猛进进出出s小说| 女女百合av大片在线观看免费| 丝袜 中出 制服 人妻 美腿| 美女高潮黄又色高清视频免费| 宝贝腿开大点我添添公交车|