国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1622KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

簡(jiǎn)要描述:KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1622
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1791

產(chǎn)品分類

Product Category

詳細(xì)介紹

KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

ATCC® Number:CRL-1622™    Price:$338.00
Designations:KLE

Depositors:GR Richardson

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:


Source:Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

Tumorigenic:Yes

Antigen Expression:blood type O; Rh+

DNA Profile (STR):Amelogenin: X

CSF1PO: 13,14

D13S317: 12

D16S539: 11,12

D5S818: 9,12

D7S820: 11,12

THO1: 6,7

TPOX: 8,11

vWA: 16



Age:64 years adult

Gender:female

Ethnicity:Caucasian

Comments:Electron microscopy of tumors formed in nude mice shows microvilli and junctional complexes, and nucleolar channel systems are present that are similar to those seen in normal endometrium under progestational stimulation. The tumors do not form glands.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:                    
  1. Remove and discard cult   ure medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久亚洲精品无码AV| 天天躁恨恨躁夜躁2020| 无码熟妇人妻av在线| 激情综合亚洲色婷婷五月APP| 麻豆AV一区二区三区久久| 男生把手放进我内裤揉摸好爽| 麻豆蜜桃69无码专区在线| 国内精品一区二区三区| 学长别揉了~流水了想要~| 偷拍真实夫妇作爱视频| 永久免费AV无码网站国产| 一天不日b浑身难受| 精品久久久久久亚洲精品| 人妻少妇被粗大爽9797pw| 杨敏思版1-5完整无删减版| 孩交精品XXXX视频视频| 40岁了水还特别多怎么回事| 艳鉧动漫1~6全集在线观看| app在线免费观看视频| 亚洲av激情无码专区在线播放| av动态图| 欧美乱大交XXXXX疯狂俱乐部| 国产精产国品一二三产区区别| 国产成人亚洲精品无码H在线| 性裸交a片a∨天传媒公司| 在线高清日韩大全免费观看| spy3wc撒spy3wc撒尿| 欧美人伦禁忌DVD放荡欲情| 马鞍山师范高等专科学校| 国产精品久久一区二区三区蜜桃| 国99精品无码一区二区三区| 国产又色又爽又黄又刺激视频国语| 成熟丰满熟妇AV无码区| av超薄肉色丝袜交足视频| 日韩AV无码一区二区三区不卡| 装醉把自己给流浪汉玩| 国精品人妻无码一区二区三区软件| 久久精品国产亚洲AV麻豆| 久久婷婷五月综合国产尤物APP| 把老熟妇日出白浆| 久久天天躁狠狠躁夜夜AV浪潮|