国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-2541C8-D1A 小鼠小腦細(xì)胞

C8-D1A 小鼠小腦細(xì)胞

簡(jiǎn)要描述:CRL-2541 C8-D1A 小鼠小腦細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2541
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2715

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-2541 C8-D1A 小鼠小腦細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-2541 C8-D1A 小鼠小腦細(xì)胞

ATCC® Number: CRL-2541™    Price: $339.00
Designations: C8-D1A [Astrocyte type I clone]

Depositors: B Pessac, D Trisler

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:neuronal


Source:Organ: brain
Strain: C57BL/6
Tissue: cerebellum


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2541

Cytogenetic Analysis:pseudodiploid, pseudodiploid [48963]

Age: 8 days

Comments:Clonal permanent cell lines with astrocytic or microglial properties have been established from explant cultures of 8-day postnatal mouse cerebella after in vitro spontaneous transformation. [48963] [49701]

Sister flasks of lethally irradiated astrocytes, which are known to synthesize the macrophage-microglia growth factor, M-CSF, were used as a substrate for cloning the cells. [48963] [49701]

The cell lines were derived in a multistage process. Slowly proliferating foci with several morphologies appeared 4 months after initiation of the cultures and became progressively enriched by cells with a homogeneous appearance. [48963]

Some of these cloned cell lines bound anti-glial fibrillary acidic protein (GFAP) antibodies and therefore appeared to be astrocytic. No other glial neuronal or microglial markers have been detected in these clones. [48963]

According to their morphology, 3 separate types of these GFAP-positive clones could be distinguished. Type I and II cells had small somata. [48963]

Type I had several short processes, while type II had two processes, one of which was very thin and long (greater than 200 microns). Type III cells had large flat somata and no processes. [48963]

The astrocyte type II cloned cell line named C8-S is available as ATCC CRL-2535 and the astrocyte type III cloned cell line named C8-D30 is available as ATCC CRL-2534.

One clone with microglial properties named C8-B4 is available as ATCC CRL-2540.

The C8-D1A cell line has the morphology of fibrous astrocytes.

These astrocytic clones might be the in vitro counterparts of fibrous (type I), or velamentous (type III) astrocytes and of Golgi epithelial cells (type II). [48963]



Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days


Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:48963: Alliot F, Pessac B. Astrocytic cell clones derived from established cultures of 8-day postnatal mouse cerebella. Brain Res. 306: 283-291, 1984. PubMed: 6466977

49701: Alliot F, et al. A spontaneously immortalized mouse microglial cell line expressing CD4. Brain Res. Dev. Brain Res. 95: 140-143, 1996. PubMed: 8873987




















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
末成年女A∨片一区二区| 性色AV一区二区三区无码| 性xxxx视频播放免费| 小说区 图片区色 综合区| 人妻互换亂倫激情| 最美情侣视频免费观看完整版| 亚洲国产精品成人AV在线| 欧美变态深喉囗交xxxx| 国产又色又爽又黄又刺激视频国语| 亚洲AV成人中文无码专区| 久久国产精品亚洲AV四虎| 美女性爽视频国产免费app| 差差漫画在线观看登录页面弹窗| 狠狠色噜噜狠狠狠888米奇视频| 久久久久久精品免费免费WEI| 年轻又漂亮的后妈2| 国产成人无码综合亚洲日韩| 妺妺窝人体色WWW看人体| 俄罗斯ve乳librederm| 日产精品一线二线三线芒果| 亚洲日本一区二区三区在线不卡| 他强把手指伸进我的下面| 他一边曰一边吃我奶小说免看| 欧美乱妇狂野欧美在线视频| 天天做天天爱夜夜爽毛片毛片 | 嫩模被强到高潮呻吟不断| 黄页网站推广app天堂| 熟妇高潮精品一区二区三区| 激情偷乱人伦小说免费看| 免费又黄又爽又色的视频| 寡妇玩xxxxxx猛男| 亚洲人成无码网站久久99热国产| 久久天天躁夜夜躁狠狠85麻豆| 久久99热狠狠色精品一区| 国产毛毛浓密茂盛| 苍井空亚洲精品aa片在线播放| 含着她的花蒂咬到高潮| 国产肥熟女视频一区二区| 香蕉久久精品日日躁夜夜躁| 奇米777狠狠888俺也去| 永久免费AV无码网站性色AV|